Journal: Bioactive Materials

Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

doi: 10.1016/j.bioactmat.2026.02.051

Figure Lengend Snippet: In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 °C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark.

Techniques: In Vitro, Fluorescence, Staining

Journal: Cell Reports Medicine

Article Title: Targeting TPX2-dependent lineage plasticity by CDK4/6 inhibition reverses therapy resistance in neuroendocrine bladder carcinoma

doi: 10.1016/j.xcrm.2026.102712

Figure Lengend Snippet: Single-cell transcriptomics reveals heterogeneity of the cell cycle dynamics underlying NE lineage plasticity (A and B) UMAP visualization of single-cell transcriptomes from the in-house (A, 64,756 cells) and public (B, 201,720 cells) scRNA-seq cohorts, colored by major cell type annotations. The zoomed views show the UMAP visualizations of epithelial cells, classified into lu/ba, NE-like, and NE subtypes. (C) Dot plot showing the expression patterns of lu/ba, NE-like, and NE signature genes across epithelial cell subtypes from the in-house and public cohorts. (D) KEGG pathway enrichment analysis of DEGs from in-house scRNA-seq cohort, showing the top enriched pathways for NE-like vs. lu/ba epithelial cells and NE vs. lu/ba epithelial cells, with cell cycle-related pathways prominently enriched in both comparisons. (E) NMF-based similarity matrix of integrated epithelial cells from both in-house and public scRNA-seq cohorts was constructed with NE differentiation-associated genes from the MEmidnightblue module were identified via WGCNA. (F) UMAP visualization of epithelial cells from (E) colored by dominant MP assignment. (G) Heatmap showing the observed-to-expected ratio (R o/e) of meta-program-assigned epithelial cells across lu/ba, NE-like, and NE subtypes. (H) Sankey diagram showing the correspondence between NE/NE-like subtype annotations and dominant MP assignments. NE-like cells are predominantly associated with MP1, whereas NE-like cells align with MP2. (I) GSEA plot showing the enrichment scores for the G2/M and G1/S cell cycle phases for the MP1 and MP2 groups. NES, normalized enrichment score. (J) Cell cycle phases of lu/ba, NE-like, and NE epithelial cells from the in-house and public scRNA-seq cohorts.

Article Snippet: Cell cycle analysis kit , MultiScience , Cat#CCS012.

Techniques: Single-cell Transcriptomics, Single Cell, Expressing, Construct

Journal: Cell Reports Medicine

Article Title: Targeting TPX2-dependent lineage plasticity by CDK4/6 inhibition reverses therapy resistance in neuroendocrine bladder carcinoma

doi: 10.1016/j.xcrm.2026.102712

Figure Lengend Snippet: Mapping reveals TPX2 as a representative and functionally relevant driver of NE-like cells (A) Violin plot showing correlations between NE-like cell top markers and the NE-like Sig score across multiple BLCA transcriptomic cohorts. TPX2, highlighted in red, shows the strongest correlation among the markers analyzed. (B and C) Spatial transcriptomic analysis showing the expression of TPX2 and NE-like Sig scores across spatial locations in bladder tumor tissue (B) and their positive correlation (C). R indicates the Spearman correlation coefficient. (D) Representative mIF image of NEBC tissue showing TPX2 (magenta), INSM1 (cyan), and DAPI (blue). The inset highlights TPX2 + (red arrowheads) and INSM1 + (white arrowheads) cells, which demonstrated minimal colocalization. Scale bars, 50 μm. (E and F) Representative mIF images (E) and quantification (F) showing the proportion of TPX2 + panCK + tumor cells across different tumor grades (Tis/Ta/T1: n = 55; T2: n = 46; T3: n = 43; T4: n = 35). Scale bars, 50 μm. (G and H) Flow cytometry (G) and quantification (H) showing the proportions of T24 and UM-UC-3 cells in different cell cycle phases 48 h after transfection with control siRNA or TPX2 siRNA ( n = 3 per group). (I and J) Flow cytometry (I) and quantification (J) showing the proportions of T24 and UM-UC-3 cell lines in different cell cycle phases in the empty vector control and TPX2-oe groups ( n = 3 per group). (K) qPCR analysis was performed to evaluate changes in the expression levels of NE markers following TPX2 knockdown in the T24 cell line. The data are presented as the means ± SDs in (H, J, and K). The box inside violin plot (A) represents the interquartile range (IQR), with the middle line as the median and the hinges corresponding to the 25th and 75th percentiles. p values were determined by one-way ANOVA in (F, H, and K) and by two-tailed unpaired Student’s t test in (J).

Article Snippet: Cell cycle analysis kit , MultiScience , Cat#CCS012.

Techniques: Expressing, Flow Cytometry, Transfection, Control, Plasmid Preparation, Knockdown, Two Tailed Test